Table 1. Comparative characteristics of electrochemical systems.
Bioobject(biomolecule) |
Electrode modification |
Electrochemical method, mode of experiment, type of process |
Linear range |
Cytochrome P450
|
SPE, membrane-like compounds DDAB, Au nanoparticles/DDAB sodium montmorillonite colloid (Na,Ca)0.33(Al,Mg)2(Si4O10) and Pt nanoparticles |
CV, amperometry, SWV, vertical mode, Protein film voltammetry |
Γ=10-15 mol /electrode* |
Proteins and peptides without prostetic groups |
SPE/MWCNT |
SWV, DPV, Horizontal mode; Diffusion-controlled |
1 nM ÷0.1 mM |
DNA |
SPE/MWCNT |
SWV, DPV, Horizontal mode; Diffusion-controlled |
5 ÷ 500 μg/ml, Guanine oxidation, Е=+0.7±0,05 V 0.5 ÷ 50 μg/ml, Adenine oxidation, Е=+0.96±0.05 V |
Acetaminophen |
SPE/MWCNT |
SWV, DPV, Horizontal mode; Diffusion-controlled |
100 nM ÷5 mM |
*the quantity of electroactive cytochrome P450 was calculated by means of DPV