Table 1. ELISA conditions for the detection of natural antibodies to GABA and glutamic acid in analyzed serum blood samples.
|
Test antigen sorbed on solid phase |
Isotype of immunoglobulins |
Serum dilution interval |
The concentration range of the sorbed antigen, ug/ml |
|
GABA |
IgM |
1/200 – 1/800 |
0.5 – 2 |
|
IgG |
1/100 – 1/600 |
0.5 – 2 |
|
|
Glutamate |
IgM |
1/100 – 1/400 |
1 – 3 |
|
IgG |
1/100 – 1/200 |
0.5 – 2 |