Table 1. Immobilization protocol for Mab to B2M. Executed commands were derived from the queue window Biacore 3000 Control Software.
|
Time, s |
Info |
|
0 |
Timestamp: 14-May-18 10:51:05 |
|
0 |
Set Temperature: 25.0°C |
|
0 |
Temperature: 25.0°C |
|
0 |
Detection Mode: Single |
|
0 |
Rack 1 (Left): THERMO_C |
|
0 |
Rack 2 (Right): THERMO_A |
|
0 |
Rack R (Reagent): Reag_A |
|
0 |
Flow Cell: 2 |
|
0 |
Flow: 10 μl/min |
|
0 |
Data Collection: 1 Hz |
|
616.3 |
Start QUICKINJECT |
|
649.1 |
Inject: Start Fc 2 R2A1 |
|
1068.8 |
Inject: Ready 70 μl |
|
1103.5 |
End QUICKINJECT |
|
1164.5 |
Start QUICKINJECT |
|
1197.3 |
Inject: Start Fc 2 R2A1 |
|
1616.9 |
Inject: Ready 70 μl |
|
1652.2 |
End QUICKINJECT |
|
1723.4 |
Start QUICKINJECT |
|
1756.2 |
Inject: Start Fc 2 R2A1 |
|
2175.9 |
Inject: Ready 70 μl |
|
2210.4 |
End QUICKINJECT |
|
2336.5 |
Temperature: 25.0°C |