Table 2. Protocol of SPR registration of protein – protein interactions. Executed commands were derived from the queue window Biacore 3000 Control Software.
Time, s |
Info |
0 |
Timestamp: 14-May-18 14:00:24 |
0 |
Set Temperature: 25.0°C |
0 |
Temperature: 24.99°C |
0 |
Detection Mode: 1,2 |
0 |
Rack 1 (Left): THERMO_C |
0 |
Rack 2 (Right): THERMO_A |
0 |
Rack R (Reagent): Reag_A |
0 |
Flow Cell: 1-2 |
0 |
Flow: 10 μl/min |
0 |
Data Collection: 1 Hz |
939.4 |
Transfer: Start 450 μl B->R2A1 |
1023.5 |
Transfer: Ready |
1587 |
Start QUICKINJECT |
1619.9 |
Inject: Start Fc 1-2 R2A1 |
1919.7 |
Inject: Ready 50 μl |
1955.6 |
End QUICKINJECT |
2147.1 |
Transfer: Start 450 μl B->R2A2 |
2231.2 |
Transfer: Ready |
2690.9 |
Start QUICKINJECT |
2721.1 |
Flowfill: Start |
2729.1 |
Flowfill: Ready |
2761.3 |
Inject: Start Fc 1-2 R2A1 |
2790.8 |
Inject: Ready 5 μl |
2824.1 |
End QUICKINJECT |
2938.4 |
Start QUICKINJECT |
2971.7 |
Inject: Start Fc 1-2 R2B1 |
3271.6 |
Inject: Ready 50 μl |
3307.4 |
End QUICKINJECT |
3347 |
Start QUICKINJECT |
3380 |
Inject: Start Fc 1-2 R2C1 |
3559.6 |
Inject: Ready 30 μl |
3594.4 |
End QUICKINJECT |
3624.6 |
Start QUICKINJECT |
3655.7 |
Inject: Start Fc 1-2 R2A1 |
3685.2 |
Inject: Ready 5 μl |
3718.1 |
End QUICKINJECT |
4149.3 |
Transfer: Start 450 μl B->R2A1 |
4233.6 |
Transfer: Ready |
4382.7 |
Start QUICKINJECT |
4416 |
Inject: Start Fc 1-2 R2B1 |
4715.8 |
Inject: Ready 50 μl |
4751.4 |
End QUICKINJECT |
4752.4 |
Start QUICKINJECT |
4784.7 |
Inject: Start Fc 1-2 R2C1 |
4964.4 |
Inject: Ready 30 μl |
4998.6 |
End QUICKINJECT |
5002.5 |
Start QUICKINJECT |
5033.5 |
Inject: Start Fc 1-2 R2A1 |
5063 |
Inject: Ready 5 μl |
5095.5 |
End QUICKINJECT |
5412.1 |
Start QUICKINJECT |
5442.2 |
Flowfill: Start |
5450 |
Flowfill: Ready |
5482.3 |
Inject: Start Fc 1-2 R2A1 |
5511.8 |
Inject: Ready 5 μl |
5544.7 |
End QUICKINJECT |
6391.5 |
Temperature: 25.0°C |