Table 2. Protocol of SPR registration of protein – protein interactions. Executed commands were derived from the queue window Biacore 3000 Control Software.

Time, s

Info

0

Timestamp: 14-May-18 14:00:24

0

Set Temperature: 25.0°C

0

Temperature: 24.99°C

0

Detection Mode: 1,2

0

Rack 1 (Left): THERMO_C

0

Rack 2 (Right): THERMO_A

0

Rack R (Reagent): Reag_A

0

Flow Cell: 1-2

0

Flow: 10 μl/min

0

Data Collection: 1 Hz

939.4

Transfer: Start 450 μl B->R2A1

1023.5

 Transfer: Ready

1587

 Start QUICKINJECT

1619.9

 Inject: Start Fc 1-2 R2A1

1919.7

 Inject: Ready 50 μl

1955.6

 End QUICKINJECT

2147.1

 Transfer: Start 450 μl B->R2A2

2231.2

 Transfer: Ready

2690.9

 Start QUICKINJECT

2721.1

 Flowfill: Start

2729.1

 Flowfill: Ready

2761.3

 Inject: Start Fc 1-2 R2A1

2790.8

 Inject: Ready 5 μl

2824.1

 End QUICKINJECT

2938.4

 Start QUICKINJECT

2971.7

 Inject: Start Fc 1-2 R2B1

3271.6

 Inject: Ready 50 μl

3307.4

 End QUICKINJECT

3347

 Start QUICKINJECT

3380

 Inject: Start Fc 1-2 R2C1

3559.6

 Inject: Ready 30 μl

3594.4

 End QUICKINJECT

3624.6

 Start QUICKINJECT

3655.7

 Inject: Start Fc 1-2 R2A1

3685.2

 Inject: Ready 5 μl

3718.1

 End QUICKINJECT

4149.3

 Transfer: Start 450 μl B->R2A1

4233.6

 Transfer: Ready

4382.7

 Start QUICKINJECT

4416

 Inject: Start Fc 1-2 R2B1

4715.8

 Inject: Ready 50 μl

4751.4

 End QUICKINJECT

4752.4

 Start QUICKINJECT

4784.7

 Inject: Start Fc 1-2 R2C1

4964.4

 Inject: Ready 30 μl

4998.6

 End QUICKINJECT

5002.5

 Start QUICKINJECT

5033.5

 Inject: Start Fc 1-2 R2A1

5063

 Inject: Ready 5 μl

5095.5

 End QUICKINJECT

5412.1

 Start QUICKINJECT

5442.2

 Flowfill: Start

5450

 Flowfill: Ready

5482.3

 Inject: Start Fc 1-2 R2A1

5511.8

 Inject: Ready 5 μl

5544.7

 End QUICKINJECT

6391.5

 Temperature: 25.0°C