Table 2. Kinetic parameters of the synthesized substrate hydrolysis under the thrombin action.
|
Substrate |
KM, mkM |
Vmax, mkM/s |
Vmax/ KM, s-1 |
|
Bz-FVR-pNA |
34.5 |
0.100 |
2.9 × 10-3 |
|
Bz-VPR-pNA |
35.5 |
0.034 |
9.6 × 10-4 |
|
Bz-GPR-pNA |
29.0 |
0.0136 |
4.7 × 10-4 |
|
Bz-LTPR-pNA |
134 |
1.16 |
8.7 × 10-3 |