Table 1. Parameters of the enzyme immunoassay system using different diluents.
|
|
RD6-3 |
RD5P |
PBS+4% BSA |
PBS+1% BSA |
PBS+0.1% BSA |
|
Optical density of blank sample, M ± SD |
0.030 ± 0.001 |
0.033 ± 0.002 |
0.027 ± 0.002 |
0.011 ± 0.0003 |
0.010 ± 0.0004 |
|
Standart sample, CV |
3.4 |
3.7 |
6.6 |
3.8 |
5.0 |
|
n |
24 |
24 |
24 |
24 |
24 |
|
Control sample, CV |
5.3 |
7.1 |
4.4 |
2.5 |
7.0 |
|
n |
12 |
12 |
12 |
12 |
12 |
|
LF sample, CV |
13.7 |
8.1 |
9.6 |
7.1 |
5.0 |
|
n |
24 |
24 |
24 |
24 |
24 |
|
Linear deviation for calibration curve, |
0.3714 |
1 |
0.2857 |
0.1429 |
0.2857 |
|
р-values for comparisons of calibration curve slopes between RD6-3 and the other diluents |
|
0.0001 |
0.04819 |
0.000729 |
0.0001 |
|
Linear deviation for LF sample dilution curve, |
0.6667 |
1 |
0.6667 |
1 |
1 |
Note.*p – accuracy of deviation from linearity of the Wald-Wolfowitz runs test score.