Table 2. Lacrimal CNTF concentration (pg/ml) in samples with and without acid treatment at different dilutions.
|
dilution |
|
RD6-3 |
RD5P |
PBS +4% BSA |
PBS +1% BSA |
PBS +0.1% BSA |
||||||||||
|
without acid treatment |
after acid treatment |
p |
without acid treatment |
after acid treatment |
p |
without acid treatment |
after acid treatment |
p |
without acid treatment |
after acid treatment |
p |
without acid treatment |
after acid treatment |
p |
||
|
1 |
M |
20.4 |
36.6 |
0.0005 |
18.6 |
41.8 |
0.0000 |
19.2 |
37.9 |
0.0001 |
20.3 |
39.8 |
0.0000 |
18.0 |
36.2 |
0.0000 |
|
SD |
1.1 |
2.5 |
1.2 |
1.4 |
1.7 |
1.2 |
0.9 |
1.3 |
0.6 |
0.7 |
||||||
|
CV |
5.4 |
6.9 |
6.3 |
3.4 |
8.7 |
3.2 |
4.5 |
3.3 |
3.4 |
2.0 |
||||||
|
0.5 |
M |
8.4 |
16.7 |
0.0004 |
7.4 |
18.7 |
0.0004 |
12.0 |
19.8 |
0.0053 |
11.7 |
19.5 |
0.0023 |
11.6 |
17.6 |
0.0002 |
|
SD |
0.5 |
1.2 |
0.6 |
1.7 |
1.0 |
2.2 |
0.9 |
1.7 |
0.5 |
0.7 |
||||||
|
CV |
6 |
7.1 |
8.3 |
9.1 |
8.2 |
11.3 |
8.0 |
8.8 |
4.0 |
3.7 |
||||||
|
0.25 |
M |
3.9 |
9.9 |
0.0024 |
4.6 |
12.0 |
0.0004 |
6.0 |
11.6 |
0.0008 |
5.3 |
10.4 |
0.0002 |
4.6 |
8.9 |
0.0009 |
|
SD |
0.9 |
1.3 |
0.4 |
1.1 |
0.4 |
1.0 |
0.5 |
0.4 |
0.3 |
0.8 |
||||||
|
CV |
22.3 |
12.7 |
9.5 |
8.9 |
6.0 |
8.8 |
9.0 |
4.2 |
5.5 |
9.2 |
||||||
|
0.125 |
M |
2.7 |
4.5 |
0.0539 |
3.2 |
5.8 |
0.0011 |
2.3 |
5.4 |
0.0013 |
2.6 |
5.3 |
0.0005 |
2.0 |
4.1 |
0.0001 |
Note. n = 6 for each measurement point.