Table 3. Reproducibility of control measurements obtained on the 0 to 250 pg/ml calibration curve with PBS and 1% BSA diluent.
|
Intra-assay precision |
Inter-assay precision |
||||||
sample |
QC20 |
QC20 |
QC20 |
LF |
QC20 |
QC20 |
QC20 |
LF |
dilution |
1 : 100 |
1 : 10 |
1 |
1 : 3 |
1 : 100 |
1 : 10 |
1 |
1 : 3 |
n |
10 |
10 |
10 |
10 |
50 |
40 |
40 |
40 |
main |
2.6 |
24.5 |
245.4 |
10.2 |
2.7 |
25.1 |
247.7 |
10.2 |
standard deviation |
0.4 |
0.9 |
3.2 |
0.6 |
0.4 |
0.8 |
6.0 |
0.5 |
CV |
13.8 |
3.6 |
1.3 |
5.4 |
15.4 |
3.4 |
2.4 |
5.2 |