Table 2. Primers used for real-time RT-PCR.
|
Target |
Forward primer (5’-3’) |
Reverse primer (5’-3’) |
Product size, b.p. |
Tann °C |
|
Casp-2 total |
GCATGTACTCCCACCGTTGA | TGCTCAACACCAGTGCTAGG |
242 |
60 |
|
Casp-2L |
AGCTCTTTGACAACGCCAA |
CAGGAACCTCGTTTGGTGTT |
262 |
58 |
|
Casp-2S |
CCGTGGAGGTGCTATTGG |
TCGGCAACTTTTCTTTACCG |
165 |
57 |
|
18S |
GGATCCATTGGAGGGCAAGT |
ACGAGCTTTTTAACTGCAGCAA |
91 |
64 |