Figure 1. Western blot analysis of culture medium from HeLa-PGLYRP1 and HeLa-PGLYRP1m cell lines. The membrane was incubated with anti-6His monoclonal antibodies. Lanes 1, 2, 3 – 50, 25, 10 ng of purified recombinant PGLYRP1, lanes 4, 5, 6 – three clones of HeLa-PGLYRP1, 7 - HeLa-PGLYRP1m, the molecular mass markers are shown on the right (kilodaltons).