Таблица 1. Influence of metal ions and some reagents on the L-asparaginase activity of RrA mutant forms.
|
Reagent |
(mM) |
% residual activities |
Reagent |
(mM) |
% residual activities |
|
KСl |
10 |
79-91 |
KMnO4 |
2 |
14-18 |
|
CaCl2 |
10 |
79-83 |
CoCl2 |
2 |
78-86 |
|
ZnCl2 |
10 |
79-88 |
Iodacetamide |
1 |
100 |
|
CuCl2 |
10 |
35-42 |
PMSF |
5 |
100 |
|
CsCl |
10 |
90-94 |
EDTA |
5 |
100 |
|
FeCl3 |
5 |
33-37 |
2-mercaptoethanol |
0.5 |
125-130 |
|
MnCl2 |
5 |
54-59 |
GSH |
1 |
139-143 |
|
MgCl2 |
2 |
129-133 |
NaN3 |
5 |
118-121 |
Note. The experiments were performed in 0.1 M Na-phosphate (pH 7.4) and 0.1 M Tris (pH 8.5) buffer. The incubation time with the reagent – 30 minutes at 4°C.