Figure 4. Typical binding sensograms of protein material from lyzate samples with immobilized on the optical chip cytochrome b₅. Next injections are indicated with numerals: (1) - lysate sample (control); (2) - lysate sample treated with 50 mM HCl (followed by subsequent neutralization) with addition of excess of exogenous CYB5A; (3) - lysate sample treated with 50 mM HCl (followed by subsequent neutralization). 1 RU (resonance unit) corresponds to 1 pg of protein bound to 1 mm² of the chip surface.