Figure 2. A. Separation of exosome proteins (Exo), secretome (Sec), and proteins retained by the sucrose cushion (Suc) obtained from the conditioned medium with (wFBS) and without FBS(w/oFBS) in a one-dimensional polyacrylamide gel electrophoresis (1D-PAGE) using the secreted protein isolation protocol according to the UF-UC-SUC scheme; BSA - bovine serum albumin (50 μg), M - molecular weight protein marker (Precision Plus Protein ™ , "Bio-Rad", USA); B and C. Extracted ion chromatogram for exosome samples obtained from conditioned medium with (wFBS) and without PBS (w/oFBS), using the UF-UC-SUC isolation protocol for secreted proteins. Samples were hydrolyzed on centrifugal filters (FASP).