Table 1. The number of proteins identified in exosome and secretome samples of Caco-2 cell line.

Sample

The total number of identified proteins

The number of identified proteins (at least 2 peptides)

Exo_wFBS_In_Solution_Digestion (p1)

230

130

Exo_wFBS_FASP(p2)

51

24

Exo_wFBS_In_Gel_Digestion(p2)

150

82

Exo_w/oFBS_FASP(p2)

291

177

Exo_w/oFBS_In_Gel_Digestion(p2)

175

89

Sec_wFBS_FASP (p2)

24

7

Sec_wFBS_In_Gel_Digestion(p2)

86

40

Sec_w/oFBS_FASP(p2)

202

160

Sec_w/oFBS_In_Gel_Digestion(p2)

132

86

 

Note. P1 is the secreted protein isolation protocol according to the UC1-UC2-SUC scheme; P2 is the secreted protein isolation protocol according to the UF-UC-SUC scheme. Complete lists of identifications are given in Supplementary materials.