Figure 1. Efficiency of transfection and EndoG induction in human, mice and rat lymphocytes. Results of flow cytometry for CD4+ T lymphocytes from human (A), mice (B) and rats (C) lymphocytes 48 h after transfection. Transfection efficiency determined by flow cytometry (D). Number of CD4+ T lymphocytes with damaged DNA, measured by TUNEL for flow cytometry (E). Induction of TUNEL-positive cells 48 h after incubation with cisplatin (F). EndoG mRNA levels in cells determined by real time RT-PCR. mRNAs levels are normalized relative to the expression of the reference 18S gene. (n = 4).