Figure 1. SPR assessment of the NADPH effect on the kinetic parameters of protein-protein interaction using CYP2E1/CPR as an example: (A) - injection of 250 nM CYP2E1 via immobilized on the chip CPR protein in the absence (solid line) or presence of 100 µМ NADPH (dotted line). (B) - injection of the working buffer (solid line) or 100 µM of NADPH (dotted line) after the formation of CYP2E1/CPR complex in the absence of NADPH. All samples were injected for 5 min at a flow rate of 10 µl/min.