Table 2. A typical experiment aimed at the detection of the modulating effect of a small compound (SC) on kinetics (affinity) of binary BBI .

<

Panel

Protein analyte * (µM) / SC (µM)

Reference

0.01 / 0

0.05 / 0

0.1 / 0

0.5 / 0

1 / 0

10 / 0

Control

0 / 10

0 / 25

0 / 50

0 / 75

0 / 100

0 / 200

Targeted №1

0.01 / 10

0.05 / 10

0.1 / 10

0.5 / 10

1 / 10

10 / 10

Targeted №2

0.01 / 25

0.05 / 25

0.1 / 25

0.5 / 25

1 / 25

10 / 25

Targeted №3

0.01 / 50

0.05/ 50

0.1 / 50

0.5 / 50

1 / 50

10 / 50

Targeted №4

0.01/ 75

0.05 / 75

0.1 / 75

0.5 / 75

1 / 75

10 / 75

Targeted №5

0.01/ 100

0.05 / 100

0.1 / 100

0.5 / 100

1 / 100

10 / 100

Targeted №6

0.01 / 200

0.05/ 200

0.1 / 200

0.5 / 200

1 / 200

10 / 200

Note. *Protein analyte was injected to the cell in solution while its partner (protein ligand) was covalently immobilized onto an optical biosensor chip.