Table 1. Primers used in PCR .

Primer

Nucleotide sequence (5' → 3')

 Mat-RNLS-for

gagctc ggatcc ga aatggaagcg ctgctgaggaggcag

Mat-RNLS-rev(+stop)

tcgtg ctcgag ctaaatataattctttaaagcttccag

Mat-RNLS-rev(-stop)

tcgtg ctcgag aatataattctttaaagcttccag

Note. BamHI, XhoI and Kozak restriction sites are shown in bold italics.