Table 1. Mass spectrometric analysis conditions for each analyte.
|
Analytes |
M1 (Da) |
M3 (Dd) |
DP (V) |
EP (V) |
CE (eV) |
CCEP (V) |
|
Estrone, quantitative transition |
504,110 |
171,100 |
56 |
10 |
43 |
14 |
|
Estrone, quality transition |
504,110 |
115,100 |
56 |
10 |
113 |
14 |
|
Estradiol, quantitative transition |
506,213 |
170,700 |
46 |
10 |
47 |
16 |
|
Estradiol, quality transition |
506,213 |
115,000 |
46 |
10 |
119 |
18 |
|
Estradiol - internal standard, quantitative transition |
511,201 |
171,100 |
101 |
10 |
45 |
20 |
|
Estradiol - internal standard, quality transition |
511,201 |
115,000 |
101 |
10 |
119 |
8 |
Note: * - mass analyser parameters: M1, M3 - ion mass in daltons, to which the first and third quadrupoles are adjusted; DP - Declustering Potential, V; EP - Entrance Potential, V; CE - Collision Energy, eV; CCEP - Collision Cell Exit Potential, V.