Table 4. Comparative characteristics of the results of the extraction of viscumin from blood serum samples prepared by precipitation and purification on Proteo Miner columns and precipitation in 1% TCA/IPA(p < 0.05, n=6)
Initial sample volume, ml |
Recovery rate of viscumin, % |
|||||||
ProteoMiner spin columns |
Precipitation with 1 % TCA/IPA |
|||||||
10 μg/ml |
5 μg/ml |
1 μg/ml |
0.5 μg/ml |
10 μg/ml |
5 μg/ml |
1 μg/ml |
0.5 μg/ml |
|
0.2 |
6.9±1.3 |
7.0±0.5 |
7.2±0.4 |
6.8±1.7 |
7.5±1.9 |
6.9±1.6 |
- |
- |
0.5 |
5.1±0.6 |
5.4±0.8 |
5.0±0.7 |
4.8±1.2 |
3.9±0.9 |
2.5±0.7 |
1.8±0.4 |
- |
1 |
4.1±0.9 |
4.7±1.1 |
3.8±0.8 |
2.7±0.6 |
2.5±0.6 |
2.2±0.5 |
1.5±0.4 |
- |