Figure 1. Analysis of the protein products obtained after expression of the vector pET-hRen(-sp) in Е. coli Rosetta (DE3) cells. Tracks: 1 – molecular weight protein markers (arrows indicate protein markers of 70 kDa, 55 kDa, 45 kDa, 35 kDa, and 25 kDa. 2 – proteins produced by the cells without IPTG stimulation; 3 – proteins produced by IPTG stimulated cells; 4 – inclusion bodies containing recombinant tRNLS; 4 – chromatographically purified tRNLS.