Figure 2. Analysis of recombinant tRNLS solubilization from inclusion bodies in the buffer containing 8 M urea. Here and in the subsequent figure a – supernatant after inclusion bodies solubilization and centrifugation; b – sediment after inclusion bodies solubilization and centrifugation. Tracks: 1 – incubation for 15 min; 2 – incubation for 30 min; 3 – incubation for 1 h; 4 – incubation for 12 h.