Table 2. Comparison of kinetic (kon, koff) and equilibrium (Kd) constants of CYP3A4 interaction with CYB5A immobilized in different ways.
|
|
kon, M-1s-1×103) |
koff, s-1×10-4) |
Kd, uM |
|
Immobilization in a planar bilayer on a chip (data taken from [9]) |
5.5±0,3 |
5.7±0.4 |
0.11±0.02 |
|
Immobilization in detergent-saturated liposomes |
8.7 |
2.9 |
0.03 |
|
Immobilization in proteoliposomes by micellar coalescence |
n/i |
n/i |
n/i |
Note. n/i - no interaction.