Figure 3. Electrophoretic analysis of ribosomal RNA fragmentation as a consequence of acquisition of collateral activity by ribonuclease Cas13a. The concentration of Cas13a is 90 nM (panel A) and 180 nM (panel B). Red electrophoregram – only Cas13a-nuclease; blue – Cas13a-nuclease and guide RNA. The guide RNA concentration is 450 nM. Samples were incubated for 1 hour at 37 °C before analysis. 1 is the leading marker; 2 is the guide RNA; 16S and 23S indicate the peaks of 16S and 23S ribosomal RNA, respectively. The abscissa is time (s); the ordinate is fluorescence (arbitrary units).