Figure 4. Effect of Marine RNase Inhibitor on ribosomal RNA fragmentation under the action of Cas13a nuclease. Panel A – in the absence of the inhibitor; panel B – in the presence of the inhibitor at 1.6 U/µl. Red electrophoregram – only Cas13a-nuclease; blue – Cas13a-nuclease and guide RNA. The concentration of Cas13a is 180 nM; the guide RNA concentration is 450 nM. The samples were incubated for 1 hour at 37°C before analysis. 1 – the leading marker; 2 – the guide RNA; 16S and 23S indicate the peaks of 16S and 23S ribosomal RNA, respectively. The abscissa is time (s); the ordinate is fluorescence (arbitrary units).