Table 1. Results of pDNA isolation. Isolation steps: AL—alkaline lysis, silica—binding with silicon dioxide, GF—gel filtration, IPA—isopropanol precipitation, Qiagen kit—Qiagen plasmid maxi kit. OD260/OD280 is the ratio of optical densities of DNA solutions at 260 nm and 280 nm.
Sample |
Isolation steps |
Quantity, mkg |
OD260/OD280 |
1 |
AL+silica+GF |
300 |
1.93 |
2 |
AL+silica |
366 |
1.89 |
3 |
AL+IPA+GF |
1005 |
1.87 |
4 |
Qiagen kit |
830 |
1.85 |