Table 1. Results of pDNA isolation. Isolation steps: AL—alkaline lysis, silica—binding with silicon dioxide, GF—gel filtration, IPA—isopropanol precipitation, Qiagen kit—Qiagen plasmid maxi kit. OD260/OD280 is the ratio of optical densities of DNA solutions at 260 nm and 280 nm.

Sample

Isolation steps

Quantity, mkg

OD260/OD280

1

AL+silica+GF

300

1.93

2

AL+silica

366

1.89

3

AL+IPA+GF

1005

1.87

4

Qiagen kit

830

1.85