Figure 3. Electrophoresis in 12% PAAG of E.coli cell proteins during expression of the chimeric RNLS gene (vector pET-PRL-RenI): 1 - total protein of E. coli cells (DE3 Rosetta strain) without induction; 2 – total protein after induction of IPTG cells; 3 – RNLS protein with the signal sequence of the PRL gene (RNLS-prl), after purification on a Ni-Sepharose column.