Figure 3. The typical kinetic curves for the cleavage of FQ-reporters due to the Cas13a collateral activity: the dependences of fluorescence on incubation time for different amounts of sRNA. The amount of sRNA in a 50 µL sample: 1 – 2.3 ng; 2 – 4.5 ng; 3 – 9 ng. Cas13a concentration – 100 nM; gRNA/Cas13a molar ration – 4.