Table 1. DNA oligonucleotides used as templates for synthesis of gRNA and sRNA. The sequence complimentary to that of T7-promoter is shown in Italian. The complimentary sequences of oligonucleotides are shown in bold.

Name

Sequence (5’ → 3’)

Product

D8R

TTTGATCATGGCTCAGATTGAACGCTGGGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCCCCTATAGTGAGTCGTATTA

gRNA

D8T

CTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCCCCTATAGTGAGTCGTATTA

sRNA