Table 1. DNA oligonucleotides used as templates for synthesis of gRNA and sRNA. The sequence complimentary to that of T7-promoter is shown in Italian. The complimentary sequences of oligonucleotides are shown in bold.
Name |
Sequence (5’ → 3’) |
Product |
D8R |
TTTGATCATGGCTCAGATTGAACGCTGGGTTTTAGTCCCCTTCGTTTTTGGGGTAGTCTAAATCCCCTATAGTGAGTCGTATTA |
gRNA |
D8T |
CTGCCGCCAGCGTTCAATCTGAGCCATGATCAAACTCTTCCCCTATAGTGAGTCGTATTA |
sRNA |