Figure 1. Sensorgrams of C. krusei CYP51 immobilization without hyperactivation (green line) and with hyperactivation (red line) on the surface of the CM5 optical chip. The arrows indicate the following additives: A - 0.2 M EDC/0.05 M NHS, B - HBS-N, C - 20 µg/ml C. krusei CYP51 in 10 mM acetate buffer pH 4.5, and the levels: 1 - sensogram level after the procedure activation, 2 - sensogram level after the activation procedure with hyperactivation, 3 - immobilized protein level, 4 - immobilized protein level under hyperactivation conditions.