Figure 1. A - Kinetics of diformazan formation in 0.2 M carbonate buffer, pH 10.8, containing 0.075 mM HBT in the presence of 1.5 mM concentration of the corresponding coenzyme: 1 - NADPH,
2 - NADH. Controls: no NBT in the buffer and the following components are added: 3 - NADPH,
4 - NADP; 5 - only HBT in the buffer and no nicotinamide coenzymes.
B - The effect of SOD on the generation of superoxide with the participation of NADH: 1- NADH is added to the buffer; 2 - the same in the presence of SOD, 0.92 µg protein/ml. Reaction conditions as in Fig. 1 A.