Table 1. Sequences of primers used. The sequences are shown from 5’- to 3’-terminus. The asterisk in the primer sequence after “t” denotes thymidine with the attached fluorescein moiety (FAM) and in the primer name – the primer with fluorescein tags. The number in parenthesis in the primer name indicates the position of the last modified thymidine from the primer 3’-end.
Forward primer |
Reverse primer |
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Primer name |
Primer sequence |
Primer name |
Primer sequence |
F |
gctggccagtttgctacctt |
R |
acctccttcagtgcgaatcat |
F*(1) |
gct*ggccagt*t*t*gct*acct*t* |
R*(1) |
acct*cct*t*cagt*gcgaat*cat* |
F*(2) |
gct*ggccagt*t*t*gct*acct*t |
R*(4) |
acct*cct*t*cagt*gcgaat*cat |
F*(6) |
gct*ggccagt*t*t*gct*acctt |
- |
- |