Figure 5. Analysis of protein products in PAGE (Coomassie R-250 staining) obtained after expression of the pET-RenRatV2 vector in E. coli Rosetta (DE3) cells. Tracks: 1) total protein of E.coli cells without induction; 2) – total protein of E. coli cells after IPTG induction; 3) – rat renalase protein with a molecular mass of 38.4 kDa after purification on a Ni-Sepharose column; 4 – molecular mass protein markers: 170 kDa, 130 kDa, 100 kDa, 70 kDa, 55 kDa, 40 kDa, 35 kDa, 25 kDa, and 15 kDa.