Table 1. Sequences of DNA oligonucleotides used as templates for the enzymatic synthesis of miRNAs and gRNAs.

Name

Sequence (5’ → 3’)

miR145-Т

agggattcctgggaaaactggacccctatagtgagtcgtatta

miR145-G

gtccagttttcccaggaatccctgttttagtccccttcgtttttggggtagtctaaatcccctatagtgagtcgtatta

miR218-T

acatggttagatcaagcacaaccctatagtgagtcgtatta

miR218-G

ttgtgcttgatctaaccatgtgttttagtccccttcgtttttggggtagtctaaatcccctatagtgagtcgtatta

miR34а-Т

acaaccagctaagacactgccaccctatagtgagtcgtatta

miR34а-G

tggcagtgtcttagctggttgtgttttagtccccttcgtttttggggtagtctaaatcccctatagtgagtcgtatta

T7F

taatacgactcactataggg

Note. The sequence of T7 RNA polymerase promoter is highlighted in bold, the sequences of gRNA spacers are underlined.