Enzyme

Substrate

Product

Limit of detection, nM

V_max, min^-1

 K_M, µМ

V_max/K_M,

min^-1/µМ

CYP2C9

(S)-Warfarin *

(S)-7-Hydroxywarfarin

91

0.100 ± 0.002

3.03 ± 0.38

0.033

CYP2C9

Diclofenac*

4ʹ-Hydroxydiclofenac

85

5.35 ± 0.13

11.66 ± 0.92

0.46

CYP2C9

(S)-Naproxene

(S)-Desmethylnaproxen

290

0.98 ± 0.03

331 ± 30

0.003

CYP2C19

Phenytoin

4-Hydroxyphenytoin

40

0.094 ± 0.002**

25.8 ± 2.0**

0.0036

CYP2E1

Chlorzoxazone*

6-Hydroxychloroisoxazone

110

1.64 ± 0.08

78 ± 9

0.021

CYP19A1

Androstenedione*

Estrone

11

51.6 ± 4.2

4.2 ± 1.5

12.29

CYP19A1

Testosterone*

β-Estradiol

3,4

10.2 ± 1.2

3.8 ± 1.3

2.68