Table 1. Primers used for PCR-based amplification of RNLS-1-9ex и RNLS-2-10ex.

Primer

Primer sequence (5’ → 3’)

Re1-9ex-forward

ATA AGA TCT ACA AAT GCT GCT GCC AAC

Re1-9ex-reverse

ATA CTG CAG TCA AAT ATA ATT CTT TAA AGC TTC CGА

Re2-10ex-forward

ATA AGA TCT CCA AGT GCT GGT GTG ATT C

Re2-10ex-reverse

ATA CTG CAG TCA GAT GGG AAA TCC AAT CGC C

Note. BglII and PstI restriction sites are shown in bold.