Table 1. Primers used for PCR-based amplification of RNLS-1-9ex и RNLS-2-10ex.
Primer |
Primer sequence (5’ → 3’) |
Re1-9ex-forward |
ATA AGA TCT ACA AAT GCT GCT GCC AAC |
Re1-9ex-reverse |
ATA CTG CAG TCA AAT ATA ATT CTT TAA AGC TTC CGА |
Re2-10ex-forward |
ATA AGA TCT CCA AGT GCT GGT GTG ATT C |
Re2-10ex-reverse |
ATA CTG CAG TCA GAT GGG AAA TCC AAT CGC C |
Note. BglII and PstI restriction sites are shown in bold.