Table 3. Catalytic parameters and zeta potential values of native RrA and its conjugates. The hydrolysis activity of 20 mM L-Asn was measured in 10 mM PBS pH 7.5 at 37 °C.
|
Sample |
KМ, mM |
Vm, IU/mg |
Zeta potential, mV |
|
RrA |
5.3 ± 1.1 |
46 ± 2 |
-11.0 ± 1.3 |
|
RrA- chitosan-PEG |
5.1 ± 1.0 |
64 ± 5 |
-5.7 ± 1.4 |
|
RrA- Chitosan-glycol |
4.1 ± 1.0 |
52 ± 3 |
0.2 ± 0.6 |
|
RrA-PEI |
3.5 ± 0.9 |
49 ± 2 |
-4.0 ± 1.8 |