Figure 1. Scheme of the design of genetically engineered structures A) PspA-sp-CheW and B) CheW-sp-PspA. Promoter T7 – strong promoter of bacteriophage T7; SD – ribosome binding site or Shine-Dalgarno box; PspA – а nucleotide sequence encoding the inter-domain region of S. pneumoniae surface antigen; CheW – а nucleotide sequence encoding a thermally stable W-type adaptive polypeptide from bacteria of the genus Thermotoga; sp – a nucleotide sequence encoding a separating glycine-serine linker; 6×His – a nucleotide sequence encoding six histidine residues; XbaI, NdeI и XhoI – recognition sites for restriction endonucleases; Terminator – terminator of transcription.