Figure 2. Control of expression of genetically engineered cassettes PspA-sp-CheW and CheW-sp-PspA in 12%SDS-PAGE in the presence of 2-Mercaptoethanol and Coomassie Brilliant Blue R-250. (the volume of samples applied to one track without thermolysis was 9 µl, with thermolysis - 16 µl). A) 1 – Molecular weight marker LRPL; 2 – E. coli BL21(DE3) pLysS [pET- CheW-sp-PspA] (induction+heating 75°С); 3 – E. coli BL21(DE3) pLysS [pET-CheW-sp-PspA] (induction, without heating); 4 – E. coli BL21(DE3) pLysS [pET-CheW-sp-PspA] (before induction+heating 75°С); 5 – E. coli BL21(DE3) pLysS [pET-CheW-sp-PspA] (before induction, without heating); 6 –E. coli BL21(DE3) pLysS (induction+heating 75°С); 7 – E. coli BL21(DE3) pLysS (induction, without heating). В) 1 – Molecular weight marker LRPL; 2 – E. coli BL21(DE3) pLysS [pET-PspA-sp-CheW] (induction+heating 75°С); 3 – E. coli BL21(DE3) pLysS [pET- PspA-sp-CheW] (induction, without heating); 4 – E. coli BL21(DE3) pLysS [pET- PspA-sp-CheW] (before induction+heating 75°С); 5 – E. coli BL21(DE3) pLysS [pET- PspA-sp-CheW] (before induction, without heating); 6 – E. coli BL21(DE3) pLysS [pET-TpeCheW] (induction+heating 75°С); 7 – E. coli BL21(DE3) pLysS [pET-TpeCheW] (induction, without heating); 8 – E. coli BL21(DE3) pLysS [pET-TpeCheW] (before induction, without heating).