Figure 4. Analysis of the soluble fraction of purified recombinant protein CheW-sp-PspA at different temperatures and pH 7.5 in 12% SDS-PAGE. 1 – Molecular weight marker LRPL; 2 – E. coli BL21(DE3) pLysS [pET-CheW-sp-PspA] (induction+heating 75°C); 3 – E. coli BL21(DE3) pLysS [pET- CheW-sp-PspA] (induction+heating 80°C); 4 – E. coli BL21(DE3) pLysS [pET-CheW-sp-PspA] (induction+heating 85°C); 5 – E. coli BL21(DE3) pLysS [pET-CheW-sp-PspA] (induction+heating 90°C); 6 – E. coli BL21(DE3) pLysS [pET-CheW-sp-PspA] (induction+heating 99°C).