Table 1. . The effect of the dosage forms (DF) of rhGM-CSF and rhGM-CSF conjugate on the peripheral blood parameters of CBA mice exposed to cyclophosphamide (CF) (5 days post cytostatic administration)
|
Animal group |
Leukocytes, 109/L |
Segmented neutrophils, 109/L |
Leukocyte formula, % |
||||
|
eosinophils |
neutrophils |
monocytes |
lymphocytes |
||||
|
band |
segmented |
||||||
|
Intact |
10.20±0.47 |
2.01±0.15 |
1.67±0.61 |
1.33±0.42 |
20.00±1.98 |
4.50±0.50 |
72.50±1.65 |
|
CF+saline solution |
1.48±0.20 |
0.08±0.02 |
0.00±0.00 |
8.00±1.71 |
5.00±0.86 |
15.33±2.29 |
71.67±3.16 |
|
CF + rhGM-CSF |
1.56±0.24 |
0.20±0.04
|
0.00±0.00 |
7.33±1.12 |
12.33±1.96 *р=0.008 |
16.00±3.43 |
64.33±4.18 |
|
CF + DF of rhGM-CSF |
2.14±0.54 |
0.28±0.09
|
0.00±0.00 |
9.33±3.04 |
12.33±2.89
|
18.00±3.72 |
60.33±5.17 |
|
Intact |
9.61±1.11 |
1.39±0.23 |
1.00±0.45 |
0.80±0.20 |
14.40±1.86 |
1.20±0.20 |
82.60±2.11 |
|
CF + saline solution |
2.49±0.14 |
0.12±0.03 |
0.00±0.00 |
7.00±1.91 |
4.83±1.30 |
10.50±0.92 |
77.67±3.43 |
|
CF + rhGM-CSF |
2.38±0.18 |
0.15±0.03 |
0.00±0.00 |
4.17±0.65 |
6.33±1.33 |
5.50±1.06 *р=0.016 |
84.00±1.59 |
|
CF + DF of rhGM-CSF conjugate |
2.63±0.22 |
0.36±0.06 *р=0.013 **р=0.013 |
0.00±0.00 |
6.00±1.32 |
13.50±1.41 *р=0.008 **р=0.012 |
9.17±1.01
|
71.33±2.25 **р=0.005 |
Note. * - differences are statistically significant compared with the parameters of mice administered with CF and saline solution, p≤0.0170; ** - differences are statistically significant compared with the parameters of mice administered with CF and rhGM-CSF substance, p≤0.0170