Table 1. The number of peptides obtained by virtual cleavage by trypsin that could theoretically match the “reference” set for different biological species.

Biological species

Number of peptides* matching the “reference” set

Total number of peptides* resulting from virtual hydrolysis

%

Homo sapiens

96552

416076

23.2%

Chlorocebus sabaeus

72635

391986

18.5%

Mus musculus

56196

366333

15.3%

Rattus norvegicus

23576

158195

14.9%

Escherichia coli

4465

53324

8.4%

Arabidopsis thaliana

581

277458

0.2%

Note. This table shows the potential number of peptides that can be detected by LC-MS/MS analysis and how many peptides can match the “reference” set. *Does not take into account modified peptides, “missing cleavage” and possible nonspecificity of hydrolysis. Peptide size from 9 to 50 amino acid residues.