Table 1. The number of peptides obtained by virtual cleavage by trypsin that could theoretically match the “reference” set for different biological species.
Biological species |
Number of peptides* matching the “reference” set |
Total number of peptides* resulting from virtual hydrolysis |
% |
Homo sapiens |
96552 |
416076 |
23.2% |
Chlorocebus sabaeus |
72635 |
391986 |
18.5% |
Mus musculus |
56196 |
366333 |
15.3% |
Rattus norvegicus |
23576 |
158195 |
14.9% |
Escherichia coli |
4465 |
53324 |
8.4% |
Arabidopsis thaliana |
581 |
277458 |
0.2% |
Note. This table shows the potential number of peptides that can be detected by LC-MS/MS analysis and how many peptides can match the “reference” set. *Does not take into account modified peptides, “missing cleavage” and possible nonspecificity of hydrolysis. Peptide size from 9 to 50 amino acid residues.