Parameter

Biacore X-100

MI-S200D

Signal units of measurement

RU (resonance units)
1000 RU ~ 1ng protein

1 RU ~ 1/10000 resonant angle (0,1 m°) [11]

m° (millidegree) (1/1000 resonant angle)
100 m° ~ 1 ng protein

1 m° ~ 10 RU

Numbers of flow cell

2

2

Baseline noise

<0.1 RU (RMS)

<1m°(RMS)

Baseline drift

<0.3 RU/min

<1m°/min

Accuracy (concentration analysis)

<5% CV

<5% CV

Number of sample tray positions

15 (1.5ml ×15)

20 (0.6 ml×8 и

1.5 ml×12)

Injection volume

5 - 90 ml

25 - 350 ml

Injection speed

1 - 100 ml/min

5 - 1000 ml/min

Temperature control

4°C - 40°C

4°C - 45°C

Unattended working hours

24 hours

60 hours

Concentration measurement

10^-11- 2∙10^-3 M

10^-10 – 10^-3 М

Association rate constant (k_on)

10³ - 10⁸ M^-1s^-1
Proteins - до 10⁸ M^-1s^-1

Low molecular weight compounds - до 10⁶ M^-1s^-1

10³ – 10⁷ M^-1s^-1

Dissociation rate constant (k_off)

10^-5 – 10^-1 s^-1

10^-5 – 10^-1 s^-1

Equilibrium dissociation constant (K_d)

10^-12 – 10^-3 М

10^-12 – 10^-4 М