Table 2. Kinetic parameters (kon and koff) and equilibrium dissociation constant Kd of antibody complexes with immobilized protein A.
|
Biosensor, chip type |
Antibody |
kon, M-1c-1 |
koff, с-1 |
Kd, M |
|
Biacore X-100, CM5 |
IgG2а |
1.69∙105 |
0.52∙10-3 |
3.08∙10-9 |
|
Inter-Bio MI-S200D, IB-CM5 |
1.65∙105 |
1.05∙10-3 |
6.36∙10-9 |
|
|
Biacore X-100, CM5 |
IgG1 |
1.56∙105 |
2.32∙10-2 |
1.49∙10-7 |
|
Inter-Bio MI-S200D, IB-CM5 |
1.95∙105 |
2.29∙10-2 |
1.17∙10-7 |