Table 1. Protective activity of the recombinant proteins of P. aeruginosa when used as an adjuvant of aluminum hydroxide and chitosan.
|
Recombinant proteins and dose (µg) |
Adjuvant and dose (µg)/p> |
Infection dose (mln m.c.) |
Number of died/survived mice |
LD50 (mln m.c.) |
IF* |
|
OprF 25 |
aluminum hydroxide 75 |
200 |
10/0 |
61.6 |
1.9 |
|
50 |
3/9 |
||||
|
12.5 |
1/9 |
||||
|
toxoid 50 |
aluminum hydroxide 150 |
200 |
9/1 |
72.4 |
2.2 |
|
50 |
5/10 |
||||
|
12.5 |
0/10 |
||||
|
OprF 25 toxoid 50 |
aluminum hydroxide 225 |
200 |
3/7 |
114.9 |
3.5 |
|
50 |
3/12 |
||||
|
12.5 |
0/10 |
||||
|
OprF 25 |
chitosan 100 |
200 |
8/2 |
63 |
1.9 |
|
50 |
2/13 |
||||
|
12.5 |
4/6 |
||||
|
toxoid 50 |
chitosan 100 |
200 |
2/13 |
83.1 |
2.5 |
|
50 |
2/9 |
||||
|
12.5 |
3/7 |
||||
|
OprF 25 |
chitosan 100 |
200 |
7/3 |
104.7 |
3.2 |
|
50 |
4/11 |
||||
|
12.5 |
0/10 |
||||
|
non-immunized mice |
100 |
9/1 |
33 |
- |
|
|
25 |
4/6 |
||||
|
6.25 |
0/10 |
||||
*IE – Indices of effectiveness of protective properties