Table 2. Protective activity of the recombinant proteins of P. aeruginosa when using different amounts of the preparations at the second immunization.
|
Recombinant proteins and dose (µg) Adjuvant and dose (µg) |
Infection dose (mln m.c.) |
Number of died/survived mice |
LD50 (mln m.c.) |
IE* |
|
|
First immunization |
Second immunization |
||||
|
OprF 25 aluminum hydroxide 25 |
OprF 25 aluminum hydroxide 25 |
200 |
10/1 |
32.2 |
2.0 |
|
50 |
6/5 |
||||
|
12.5 |
4/7 |
||||
|
OprF 25 - |
200 |
10/2 |
35.4 |
2.2 |
|
|
50 |
6/6 |
||||
|
12.5 |
5/7 |
||||
|
OprF 12.5 aluminum hydroxide 12.5 |
200 |
12/0 |
28.1 |
1.7 |
|
|
50 |
8/4 |
||||
|
12.5 |
3/9 |
||||
|
toxoid 50
aluminum hydroxide 50 |
toxoid 50 aluminum hydroxide 50 |
200 |
10/1 |
36.5 |
2.2 |
|
50 |
7/4 |
||||
|
12.5 |
2/9 |
||||
|
toxoid 50 - |
200 |
9/2 |
36.5 |
2.2 |
|
|
50 |
8/3 |
||||
|
12.5 |
2/9 |
||||
|
toxoid 25 aluminum hydroxide 25 |
200 |
11/1 |
35.4 |
2.2 |
|
|
50 |
7/4 |
||||
|
12.5 |
3/9 |
||||
|
OprF 25 chitosan 50 |
OprF 25 chitosan 50 |
200 |
9/2 |
36.5 |
2.2 |
|
50 |
5/6 |
||||
|
12.5 |
5/6 |
||||
|
OprF 25 - |
200 |
8/2 |
42,9 |
2.6 |
|
|
50 |
7/3 |
||||
|
12.5 |
1/8 |
||||
|
OprF 12.5 chitosan 50 |
200 |
10/1 |
32.2 |
2.0 |
|
|
50 |
8/3 |
||||
|
12.5 |
2/9 |
||||
|
toxoid 50 µg chitosan, 50 µg |
toxoid 50 chitosan 50 |
200 |
8/3 |
47.0 |
2.9 |
|
50 |
7/4 |
||||
|
12.5 |
2/9 |
||||
|
toxoid 50 - |
200 |
9/2 |
53.3 |
3.2 |
|
|
50 |
4/7 |
||||
|
12.5 |
3/8 |
||||
|
toxoid 25 chitosan 50 |
200 |
8/3 |
47.0 |
2.9 |
|
|
50 |
7/4 |
||||
|
12.5 |
2/9 |
||||
|
non-immunized mice |
100 |
10/0 |
16.5 |
- |
|
|
25 |
8/2 |
||||
|
6.25 |
0/10 |
||||
*IE – Indices of effectiveness of protective properties