Figure 3. Distribution of DUSP9 protein with a molecular weight of 42 kDa among urine fractions. Each lane contains proteins extracted from 15 ml of urine. 1-2 and 3-4 are single-step centrifugations of urine at 10000 g and 400 g, respectively. 5-6 – sequential centrifugation: 400 g →2000 g →10000 g →100000 g. CD9 is an exosome marker. LB - Laemmli buffer, RB – RIPA buffer.