Table 1. Antioxidant activity of the studied compounds in the enzymatic reaction xanthine-xanthine oxidase.

Substances

Concentration, мМ

 Rate,

(∆opt.density, 560nm/min)

АОА, arb.units 

 

Control

-

0.0178 ± 0.0011

-

NADPH

1.0

0.0096±0.0017*

42.8±9.8

NADP+

1.0

0.25

0.0016±0.0005*

0.0037±0.0015*

91.3±2.1

79.5±3.5

NADH

 

1.0

2.0

0.0090±0.0013*

0.0071±0.0015*

54.1±6.2

60.8±3.2

Nicotinamide

1.0

2.0

0.0103±0.0014*

0.0039±0.0016*

37.7±3.7

77.6±4.2

Adenosine

1.0

0.0121±0.0013*

45.2±3.81

SOD

(0.46 мкг/мл)

 

0.0019±0.0001*

89.3±1.2

 

Note. Reaction conditions: 50 mM Na-carbonate buffer, pH 10.2, containing 0.4 mM EDTA; 0.025 mM HCT, 0.1 mM xanthine, xanthine oxidase 11 μg protein/ml; * significant difference p relative to control, р<0.001, р<0.01, р<0.05.