Figure 1. Representative dependences of fluorescence on reaction time during cleavage of DNA reporters by recombinant nucleases fp-AapCas12b, fp-BrCas12b, fp-BrCas12b-FNLDTA, and fp-BrCas12b-RFND. Reaction temperature: 56° (A), 64° (B), 72° (C). Curves 1, 2, 3, and 4 are fp-AapCas12b, fp-BrCas12b, fp-BrCas12b-FNLDTA, and fp-BrCas12b-RFND, respectively. The fluorescence in samples without the addition of the target DNA (F₀) was subtracted from the fluorescence measured in samples where the target was present (F).