Figure 3. Initial rate V₀ of cleavage of DNA reporters by recombinant nucleases fp-BrCas12b-FNLDTA (A), BrCas12b-RFND (B) and fp-BrCas12b (C) at different reaction temperatures. Purification of the target proteins was carried out using buffer solutions from [15]. The arithmetic means and standard deviations for three independent measurements are shown.